endometrial stromal cell line kc02 44d  (ATCC)

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Integrated computational and experimental identification of a novel ML-IAP-targeting peptide with antiproliferative activity in leukaemia cells

doi: 10.1080/14756366.2026.2724128

Figure Lengend Snippet: Antiproliferative activities of peptide-1 and its scrambled peptide in leukaemia cell lines (MOLM-13 and MV-4-11) and bone marrow stromal cells (HS-27A).

Article Snippet: The human bone marrow stromal cell line HS-27A was obtained from ATCC (Manassas, VA; Cat. No. CRL-2496).

Techniques:

Journal: British Journal of Cancer

Article Title: HDAC6 is a biomarker of leukaemic dissemination and relapse in B-ALL

doi: 10.1038/s41416-026-03539-2

Figure Lengend Snippet: a Transendothelial migration of REH cells across HUVEC monolayers toward CXCL12 (100 ng/mL, 4 h) following treatment of only REH cells with TubA (TubA; 20 µM, 1 h) or DMSO. Unpaired two-tailed Student’s t test; n = 3. b Dynamics of F-actin polymerisation in REH cells pretreated with TubA or DMSO and stimulated with CXCL12 for the indicated times. Cells were immediately fixed and stained with phalloidin. Data are shown relative to time 0 (set to 1; dotted line). Two-way ANOVA; n = 3. c Transendothelial migration of wild-type (WT) and cortactin-depleted REH cells treated with TubA (20 µM, 3 h) or DMSO toward CXCL12 (100 ng/mL, 4 h). Migrated cells were counted using a Neubauer chamber. Two-way ANOVA; n = 4. d Colonisation of HS5-MSC spheroids by REH cells after 24 h co-culture. Control cells (DMSO) were CFDA-labelled and TubA-treated cells were CTV-labelled. Colonisation was quantified by flow cytometry. Paired two-tailed Student’s t test; n = 3. e Confocal imaging and quantification of REH cells (CFDA-labelled DMSO cells in green; CTV-labelled TubA-treated cells in blue) within HS5-MSC spheroids. Representative images are shown. Scale bar = 50 µm. Paired two-tailed Student’s t test; n = 3. f Surface CXCR4 expression in non-permeabilized REH cells after TubA treatment (20 µM, 3 h) and CXCL12 stimulation. Two-way ANOVA; n = 4. g Surface and total CXCR4 expression after 24 h treatment with TubA (10 µM). Two-way ANOVA; n = 3. h Surface CD29 and CD49d (VLA-4) expression after 3 h (20 µM) and 24 h (10 µM) of TubA treatment. Two-way ANOVA; n = 3. Data are shown as MFI ± standard deviation. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns not significant.

Article Snippet: The B-ALL cell lines REH (ATCC, CRL-8286) and RS4:11 (ATCC, CRL-1873), and the stromal cell line HS5 (ATCC CRL-11882) were cultured in RPMI-1640 or DMEM (Sigma/Merck), respectively, supplemented with 10% foetal bovine serum (FBS).

Techniques: Migration, Two Tailed Test, Staining, Spheroids, Co-culture Assay, Control, Flow Cytometry, Confocal, Imaging, Expressing, Standard Deviation

Journal: British Journal of Cancer

Article Title: HDAC6 is a biomarker of leukaemic dissemination and relapse in B-ALL

doi: 10.1038/s41416-026-03539-2

Figure Lengend Snippet: a Transendothelial migration of paediatric patient–derived B-ALL cells across HUVEC monolayers toward CXCL12 (100 ng/mL, 4 h) after TubA treatment (20 µM, 3 h) or DMSO. Unpaired two-tailed Student’s t test; n = 4. b Colonisation of HS5-MSC spheroids by paediatric B-ALL cells after 24 h co-culture. Control cells were labelled with CFDA and TubA-treated cells with CTV. Paired two-tailed Student’s t test; n = 5. c Confocal imaging and quantification of paediatric B-ALL cells (CFDA-labelled DMSO cells in green; CTV-labelled TubA-treated cells in blue) within HS5-MSC spheroids. Representative images are shown. Scale bar = 50 µm. Paired two-tailed Student’s t test; n = 3. Error bars indicate standard deviation. * p < 0.05.

Article Snippet: The B-ALL cell lines REH (ATCC, CRL-8286) and RS4:11 (ATCC, CRL-1873), and the stromal cell line HS5 (ATCC CRL-11882) were cultured in RPMI-1640 or DMEM (Sigma/Merck), respectively, supplemented with 10% foetal bovine serum (FBS).

Techniques: Migration, Derivative Assay, Two Tailed Test, Spheroids, Co-culture Assay, Control, Confocal, Imaging, Standard Deviation

Journal: British Journal of Cancer

Article Title: HDAC6 is a biomarker of leukaemic dissemination and relapse in B-ALL

doi: 10.1038/s41416-026-03539-2

Figure Lengend Snippet: a HDAC6 protein levels in paired bone marrow (BM) and peripheral blood (PB) blasts from newly diagnosed (debut, n = 9) and relapsed ( n = 3) paediatric B-ALL patients. Each patient contributed matched BM and PB samples ( n = 12 paired samples). Paired two-tailed Student’s t test. b Comparison of HDAC6 levels in BM- and PB-derived blasts from newly diagnosed (debut) and relapsed patients. Unpaired two-tailed Student’s t test. c Chemotaxis of patient-derived BM blasts toward CXCL12 (100 ng/mL, 4 h). One-way ANOVA. d Chemotaxis of paired BM and PB blasts toward CXCL12. One-way ANOVA. e HDAC6 levels in adhered and non-adhered patient-derived blasts following co-culture with HS5-MSC monolayers for 24 h, shown as fold change relative to cells cultured alone (set to 1, dotted line,). One-way ANOVA; n = 2 independent experiments. f HDAC6 levels in patient-derived blasts colonising HS5-MSC spheroids (‘in’) versus non-colonising cells (‘out’). Paired two-tailed Student’s t test; n = 3. HDAC6 levels in REH cells following co-culture with HS5-MSC monolayers ( g ) or HS5-MSC spheroids ( h ). One-way ANOVA ( g ); paired two-tailed Student’s t test ( h ); n = 3. i HDAC6 expression in REH cells cultured on VCAM-1–Fc (500 ng/mL) or stimulated with CXCL12 (100 ng/mL) or Activin-A (50 ng/mL) for 24 h. One-way ANOVA; n = 3. Error bars indicate standard deviation. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns not significant.

Article Snippet: The B-ALL cell lines REH (ATCC, CRL-8286) and RS4:11 (ATCC, CRL-1873), and the stromal cell line HS5 (ATCC CRL-11882) were cultured in RPMI-1640 or DMEM (Sigma/Merck), respectively, supplemented with 10% foetal bovine serum (FBS).

Techniques: Two Tailed Test, Comparison, Derivative Assay, Chemotaxis Assay, Co-culture Assay, Cell Culture, Spheroids, Expressing, Standard Deviation

Journal: British Journal of Cancer

Article Title: HDAC6 is a biomarker of leukaemic dissemination and relapse in B-ALL

doi: 10.1038/s41416-026-03539-2

Figure Lengend Snippet: a Representative immunoblot and densitometric quantification of HDAC6 in REH cells transduced with three independent HDAC6-targeting shRNAs (A, C, D) or scramble control (Scr). Acetylated tubulin and γ-tubulin served as functional and loading controls, respectively; n = 3. b Transendothelial migration of Scr and HDAC6-depleted REH cells treated with TubA (20 µM, 3 h) or DMSO toward CXCL12 (100 ng/mL, 4 h). Two-way ANOVA; n = 4. c Confocal imaging and quantification of HDAC6-depleted REH cells colonising HS5-MSC spheroids after TubA or DMSO treatment. Representative images are shown. Scale bar = 50 µm. Two-way ANOVA; n = 3. Error bars indicate standard deviation. * p < 0.05; ** p < 0.01; ns not significant.

Article Snippet: The B-ALL cell lines REH (ATCC, CRL-8286) and RS4:11 (ATCC, CRL-1873), and the stromal cell line HS5 (ATCC CRL-11882) were cultured in RPMI-1640 or DMEM (Sigma/Merck), respectively, supplemented with 10% foetal bovine serum (FBS).

Techniques: Western Blot, Transduction, Control, Functional Assay, Migration, Confocal, Imaging, Spheroids, Standard Deviation